Composite
Part:BBa_K1162012
Designed by: Kathleen Miller Group: iGEM13_Utah_State (2013-09-15)
GFP with 10x His Tag
The GFP present in this part is modified from (BBa_K208000 https://parts.igem.org/wiki/index.php?title=Part:BBa_K208000), with stop codon removed to allow for full translation to 10x His Tag, BBa_K844000 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K844000). This part is useful as it allows the user to carry out gene expression studies and purify the final protein-GFP-His Tag fusion if desired. The prefix in front of the GFP is RFC 23 compatible to allow for in-frame expression of genes. Once protein expression has been demonstrated the gene can be put in front of BBa_K844000 for protein purification without the GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 523
Illegal XhoI site found at 424 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |